NILAI ZAT GIZI MAKRO DAN AKTIVITAS ANTIOKSIDAN TEMPE KEDELAI (Glycine max L.) KOMBINASI BIJI KECIPIR (Psophocarpus tetragonolobus L.)

Value of Macro Nutrients and Antioxidant Activities Soybean (Glycine max L.) Combination of Winged Bean (Psophocarpus tetragonolobus L.)

  • Christin Octavia Banobe Program studi Ilmu Gizi, Fakultas Ilmu Kesehatan, Sains dan teknologi, Universitas Dhyana Pura, Bali
  • I Gusti Ayu Wita Kusumawati Program studi Ilmu Gizi, Fakultas Ilmu Kesehatan, Sains dan teknologi, Universitas Dhyana Pura, Bali
  • Ni Ketut Wiradnyani Program studi Ilmu Gizi, Fakultas Ilmu Kesehatan, Sains dan teknologi, Universitas Dhyana Pura, Bali

Abstract

ABSTRAK 

            Dangerous diseases, such as cancer, cardiovascular disease and degenerative diseases including diabetes are the roles of oxidative stress caused by free radicals. Antioxidants are needed by the body to overcome and prevent oxidative stress. Soybean (Glycine max L.) has been studied in recent years as an antioxidant. In addition to soybeans, winged bean is also a plant that has the same activity as soybeans.The purpose of this study was to determine the value of macro nutrients and antioxidant activity of soybean tempeh products with a combination of winged beans. The soybean with a combination of winged beans is made with several combinations, namely soybean (100%: 0%), D1: C1 (80%: 20%), D2: C2 (70%: 30%), D3: C3 (50%: 50%), D4: C4 (30 %: 70%) and D5: C5 (20%: 80%). This research is an experimental research conducted at the Gajah Mada University laboratory (UGM), which contains an analysis of water content using an oven, analysis of gold content using the dry fogging method, analysis of protein content using the kjeldahl method, analysis of fat content using the soxhlet method, analysis using different methods, analysis of antioxidant activity and total flavonoids using Uv-Vis spectrophotometer. The results obtained are soybean formulation (100%: 0%) has the highest protein and fat content, respectively 27.42% and 15.94%, while the formulation D5: C5 (20%: 80%) has the highest carbohydrate content, which is 60.19%. Tempe which contains the highest antioxidant is formulation D2: C2 (70%: 30%), equal to (78.34 ± 0,20)%. Tempe which has the highest flavonoid content is soybean formulation (100%: 0%), (0.33 ± 0.03) mg QE / ml. 


Keywords: antioxidant, nutritional value, soybean, winged bean


 


ABSTRAK 

Penyakit berbahaya, seperti, kanker, penyakit kardiovaskular serta penyakit degeneratif termasuk diabetes merupakan peran dari stress oksidatif yang disebabkan oleh radikal bebas. Tubuh memerlukan antioksidan untuk mengatasi dan mencegah stres oksidatif tersebut. Kedelai (Glycine max L.) telah diteliti beberapa tahun terakhir sebagai antioksidan. Selain kedelai, kecipir juga merupakan tanaman yang memiliki aktivitas yang sama dengan kedelai. Tujuan dari penelitian ini adalah untuk menentukan kandungan nilai zat gizi makro dan aktivitas antioksidan produk tempe kedelai dengan kombinasi biji kecipir. Tempe kedelai dengan kombinasi biji kecipir dibuat dengan beberapa perlakuan, yaitu kedelai (100% : 0%), D1:C1 (80% : 20%), D2:C2 (70% : 30%), D3:C3 (50% : 50%), D4:C4 (30% : 70%) dan D5:C5 (20% : 80%). Penelitian ini merupakan penelitian eksperimental yang dilakukan dilaboratorium Universitas Gajah Mada (UGM), yang meliputi analisis kandungan kadar air menggunakan oven, analisis kandungan kadar abu menggunakan metode pengabuan kering, analisis kandungan kadar protein menggunakan metode kjeldahl, analisis kandungan lemak menggunakan metode soxhlet, analisis kandungan karbohidrat menggunakan metode by different, analisis aktivitas antioksidan dan total flavonoid menggunakan spektrofotometer Uv-Vis. Hasil yang didapatkan yaitu formulasi kedelai (100% : 0%) memiliki kandungan protein dan lemak tertinggi yang masing-masing sebesar 27,42% dan 15,94%, sedangkan formulasi D5:C5 (20% : 80%) memiliki kandungan karbohidrat tertinggi, yaitu sebesar 60,1951%. Tempe yang mengandung antioksidan tertinggi yaitu formulasi D2:C2 (70% : 30%) sebesar (78.34 ± 0.20)%. Tempe yang memiliki kandungan flavonoid tertinggi yaitu formulasi kedelai (100% : 0%), sebesar (0.33 ± 0.03) mg QE/ml.


 


Kata kunci: antioksidan, kecipir, kedelai, nilai gizi

References

Adawiah, Sukandar. D., Muawanah, A. 2015. Aktivitas Antioksidan dan Kandungan Komponen Bioaktif Sari Buah Namnam. Jurnal Kimia Valensi 1(2) :130-136.

Andarwulan, N., Batari, R., Sandrasari, D.A., Bolling, B., Wijaya, H. 2010. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chemistry 121 (2010) : 1231–1235.

AOAC (Association of Official Analytical Chemist). 2005. Official Methods of Analytical of The Association of Official Analytical Chemist. Washington, DC : AOAC.

Astawan, M., Wresdiyati, T., Widowati, S., Bintari, S.H., Ichani, N. 2013. Phsyco-chemical Characteristics and Functional Properties of Tempe Made from Different Soybeans Varieties. Pangan 22 (3) : 241-252.

Astuti, M., Meliala, A., Dalais, F.S., Wahlqvis, M.L. 2000. Tempe a nutritious and healthy food from Indonesia. Asia Pacific Journal of Clinic and Nutrition 9 (4) : 322-325.

Baba, S.A., Malik, S.A. 2015. Determination Of Total Phenolic and Flavonoid content, antimicrobial and Antioxidant Activity Of A Root Extract Of Arisaema jacquemontii Blume. Journal of Taibah University for Science 9 (2015): 449-454.

Baequny, A., Hartono, M., dan Harnany, AS. 2015. Efek pemberian Susu kedelai Kadar Gula Darah Penderita Diabetes Melitus Tipe 2. Informasi Kesehatan Indonesia 1(2) : 89-96.

Barhe, T.A., dan Tchouya, H.R.F. 2016. Comparative Study of the anti-oxidant activity of the total polyphenols extracted from Hibiscus Sabdariffa L., Glycine max L. Merr., yellow tea and red wine through reaction with DPPH free radicals. Arabian Journal of Chemistry 9 (2016): 1-8.

Dajanta, K., Janpum, P., Leksing, W. 2013. Antioxidant capacities, total phenolics and flavonoids in black and yellow soybeans fermented by Bacillus subtilis: A comparative study of Thai fermented soybeans (thua nao). International Food Research Journal, 20(6): 3125-3132.

Dwinaningsih, E.A. 2010. Karakteristik Kimia dan Sensori Tempe Dengan Variasi Bahan Baku Kedelai/Beras Dan Penambahan Angkak Serta Variasi Lama Fermentasi. Skripsi Sarjana. Universitas Sebelas Maret : Surakarta.

Franyoto, Y.D., Kusmita, L., Mutmainah., Angrena, R.D. 2018. Total Flavonoid Content and Formulation Antioxidant Cream Stem Of Jatropha multifida l. Journal of Physics : Conf. Series, 1025.

Garretson, L., Tyl, C., and Marti, A. 2018. Effect of Processing on Antioxidant Activity, Total Phenols, and Total Flavonoids of Pigmented Heirloom Beans. Journal of Food Quality : 1-6.

Hafiludin, 2011. Karakteristik Proksimat dan Kandungan Senyawa Kimia Daging Putih dan Daging Merah Ikan Tongkol (Euthynnus affinis). Jurnal Kelautan 4 (1).

Handayani, T. 2013. Kecipir (Psophocarpus tetragonolobus L.) Potensi Lokal yang terpinggirkan. IPTEK tanaman sayuran 001.

Hidayat, M., Soeng, S., Prahastuti, S., Patricia, T.H., Yonathan, K.A. 2014. Aktivitas Antioksidan dan Antitrigliserida Ekstrak Tunggal Kedelai, Daun Jati Belanda Serta Kombinasinya. Jurnal Bionatur 16(2):89-94.

Jendela Data dan Informasi Kesehatan, 2012. Penyakit Tidak Menular. Jakarta: Kementrian Kesehatan Republik Indonesia.

Jubaidah, S., Nurhasnawati, H., Wijaya, H. 2016. Penetapan Kadar Protein Tempe Jagung (zea mays l.) Dengan Kombinasi Kedelai (glycine max (l.) merill) Secara Spektrofotometri Sinar Tampak. Jurnal Ilmiah Manuntung 2(1): 111-119.

Karutas, E.B., 2016. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition Journal 15 (71).

Kristiningrum, E., Susanto, D.A. 2015. Soybean Tempeh Producers Capability in Implementing SNI 3144:2009. Jurnal Standardisasi 16 (2): 99 – 108.

Kunsah, B. 2017. Analisa Kadar Protein Pada Teripang (holothuria argus) Terhadap Lama Perebusan. The Journal of Muhamadiyah Medical Laboratory Technologist 2(1): 23-30.

Majewska, M., Skrzycki, M., Podsiad, M., czeczot, H. 2011. Evaluation of Antioxidant Potential of Flavonoids: an in vitro study. Acta Pol Pharm-Drug Research 68 (4): 611 – 615.

Martinez, C.J., Martinez, A.C., Ayala, A.L.M., Muzquis, M., Pedrosa, M.M., Ortiz, G.D. 2012. Changes in Protein, Nonnutritional Factors, and Antioxidant Capacity during Germination of L.campestris Seeds. International Journal of Agronomy, (7).

Molyneux, P. 2004. The Use Of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) For Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol 26 (2): 211-219.

Nugraheni, K., dan Bintari, S.H. 2016. Aktivitas antidislipidemia tepung tempe dan susu kedelai pada profil lipid tikus diabetes yang diinduksi streptozotocin. Jurnal Gizi dan Dietetik Indonesia 4(3): 147-153.

Olaiya, C.O., Soetan, K.O., Karigidi, K.O., 2018. Evaluation of In Vitro Antioxidant Capacities of Six Accessions of Winged Beans (Psophocarpus tetragonolobus). EC Nutrition 13(8):589-595.

Pagara, H. 2011. Pengaruh Lama Perebusan terhadap Kadar Protein Tempe Kacang Tunggak (Vigna Unguiculata). Bionature 12(1) : 15-20.

Rusita, Y.D., Suhartono. 2016. Flavonoids content in extracts secang (Caesalpinia Sappan L.) maceration method infundation analysis and visible ultraviolet spectrophotometer. International Journal of Medical Research & Health Sciences 5 (4) : 176-181.

Saleh, M.A., Clark, S., Woodard, B., Deolu-Sobogun, S.A. 2010. Antioxidant and free radical scavenging activities of essential oils. Ethnicity & Disease 20.

Santi, R.A., Sunarti, T.C., Santoso, D., Triwisari, D.A. 2012. Komposisi Kimia dan Profil Polisakarida Rumput laut Hijau. Jurnal Akuatika 2 (2) : 105 – 114.

Sari, K.P., Jamaluddin., Sukainah, A. 2016. Fortifikasi Tempe Berbahan Dasar Kedelai dan Biji Nangka. Jurnal Pendidikan Teknologi Pertanian 2: 16-26.

Sokamte, T.A., Mbougueng, P.D., Tatsadjieu, N.I., Sachindra, N.M. 2019. Phenolic Compounds Characteriszation and Antioxidant Activities Of selected Spices from camoroon. South African Journal Of Botany, 121 : 7-15.

Sulistiani, H.R., Handayani, S., dan Pangastuti, A. 2014. Karakterisasi senyawa bioaktif isoflavon dan uji aktivitas antioksidan dari ekstrak etanol tempe berbahan baku kedelai hitam (Glycine soja), koro hitam (Lablab purpureus), dan koro kratok (Phaseolus lunatus). Biofarmasi 12 (4) : 62-72.

Utari, D.M., 2010. Kandungan Asam Lemak, Zink, dan Copper Pada Tempe, Bagaimana Potensinya Untuk Mencegah Penyakit Degeneratif?. Gizi Indonesia 33 (2): 108-115.

Wardiah, Samingan, Putri, A. 2016. Uji Preferensi Tempe Kacang Tunggak (Vigna unguiculata (l.) walp.) Yang Difermentasi Dengan Berbagai Jenis Ragi. Jurnal Agroindustri 6 (1): 34-41.

Yoon, Gun-Ae dan Park, S. 2014. Antioxidant action of soy isoflavones on oxidative stress and antioxidant enzyme activities in exercised rats. Nutrition Research and Practic 8(6): 618-624.

Yusof, H.M., Ali, N.M., Yeap, S.K., Ho, W.Y., Beh, B.K., Koh, S.P., Long, K., Aziz, S.A., Altheen, N.B. 2013. Hepatoprotective Effect of Fermented Soybean (Nutrient Enriched Soybean Tempeh) against Alcohol-Induced Liver Damage in Mice. Hindawi Publishing Corporation 8 : 274-274.
Published
2019-11-28
How to Cite
BANOBE, Christin Octavia; KUSUMAWATI, I Gusti Ayu Wita; WIRADNYANI, Ni Ketut. NILAI ZAT GIZI MAKRO DAN AKTIVITAS ANTIOKSIDAN TEMPE KEDELAI (Glycine max L.) KOMBINASI BIJI KECIPIR (Psophocarpus tetragonolobus L.). Pro Food, [S.l.], v. 5, n. 2, p. 486-495, nov. 2019. ISSN 2443-3446. Available at: <http://profood.unram.ac.id/index.php/profood/article/view/111>. Date accessed: 30 sep. 2020. doi: https://doi.org/10.29303/profood.v5i2.111.

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