UJI AKTIVITAS ANTIOKSIDAN SOSIS ANALOG TEMPE DENGAN PENAMBAHAN TEPUNG UBI JALAR UNGU TERHADAP PENURUNAN KADAR GULA DARAH MENCIT DIABETES

Antioxidant Activity Test of Tempeh Analog Sausage by Addition of Purple Sweet Potato Flour on the Decreasing of Blood Glucose Level in Diabetic Mice

Abstract

ABSTRACT

This research was aimed to observe the effect of the addition of purple sweet potato flour in the making of tempeh analog sausage on antioxidant activity, anthocyanin total, reducing sugar content and antioxidant capability on decreasing of blood glucose level in diabetic mice. The method that was used in this research was Completely Randomized Design (CRD) which consist of five treatments i.e. 0%; 10%; 15%; 20% and 25% addition of purple sweet potato flour by four replications. The data were analyzed by Analysis of Variance (ANOVA) at α = 5% using Co-stat and SPSS 21 for windows software by further analyzed Polynomial Orthogonal Method (POM). The results showed that the addition of purple sweet potato flour treatments gave significant effect on antioxidant activity, anthocyanin total, and decreasing of blood glucose level in diabetic mice which feed by the tempeh analog sausage, however gave non significant effect on the reducing glucose content of the tempeh analog sausage. The 25% addition of  purple sweet potato flour was the best treatment by 31.44% of antioxidant activity; 8.22 mg/L of anthocyanin total; 1.13% of reducing glucose content and could decrease blood glucose level in diabetic mice equal to 387 mg/dL.

Keywords : anthocyanin, blood glucose, purple sweet potato, tempeh analog sausage.

ABSTRAK

Penelitian ini bertujuan untuk menguji pengaruh penambahan tepung ubi jalar ungu pada pembuatan sosis analog tempe terhadap aktivitas antioksidan, total antosianin, kadar gula reduksi dan kemampuan antioksidannya untuk menurunkan kadar gula darah mencit diabetes. Metode yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari lima perlakuan yakni penambahan tepung ubi jalar ungu 0%, 10%, 15%, 20% dan 25% dengan 4 kali ulangan. Hasil pengamatan dianalisis menggunakan Analysis of Variance (ANOVA) pada α = 5% menggunakan software Co-Stat dan SPSS 21 for windows dengan uji lanjut menggunakan metode ortogonal polinomial (MOP). Hasil penelitian menunjukkan bahwa perlakuan penambahan tepung ubi jalar ungu memberikan pengaruh yang signifikan terhadap aktivitas antioksidan, total antosianin dan penurunan kadar gula darah mencit diabetes yang diberi makan sosis analog tempe, namun memberikan pengaruh yang tidak signifikan terhadap kadar gula reduksi sosis analog tempe. Penambahan 25% tepung ubi jalar ungu merupakan perlakuan terbaik dengan karakteristik aktivitas antioksidan 31,44%; total antosianin 8,22 mg/L; kadar gula reduksi 1,13% dan mampu menurunkan kadar gula darah mencit diabetes sebesar 387 mg/dL.

Kata kunci : antosianin, gula darah, sosis analog tempe, ubi jalar ungu