KAJIAN MUTU KIMIA DAN DAYA HAMBAT MADU LEBAH TRIGONA (Tetragonula clypearis) DARI PETERNAKAN DI KABUPATEN LOMBOK TIMUR TERHADAP Pseudomonas aeruginosa SECARA IN VITRO

Study On Chemical Quality And Inhibitory Efficacy Of Trigona Bee (Tetragonula Clypearis) Honey From Breeders In East Lombok District Against Pseudomonas Aeruginosa Using In Vitro Analysis

Authors

  • Solatul Hifzi Ulan Nasri Universitas Mataram
  • Sri Widyastuti Universitas Mataram
  • Mutia Devi Ariyana University of Mataram

DOI:

https://doi.org/10.29303/profood.v9i1.289

Keywords:

chemical quality, inhibition activity, Pseudomonas aeruginosa, trigona honey, Tetragonula clypearis

Abstract

The chemical properties and antimicrobial activity of honey can be affected by geographic location. The aim of this study was to determine the chemical quality and inhibition activity of trigona honey bee (Tetragonulaclypearis) from Breeders in East Lombok District against Pseudomonas aeruginosa in vitro. This study was conducted using completely randomized design (CRD) with one factor, namely the location of the trigona beekeeping which consisted of 6 (six) locations, namely breeders Dames Damai, Bintang Rinjani, Sukarema, Bebidas, Banjar Sari and Geres.). Data were analyzed by analysis of variance (ANOVA) at a significance level of 5% using Co-Stat software. Significantly different data were tested with an Honestly Significant Difference (HSD). Data of phytochemical compounds (tanins and flavonoids) were analyzed using a descriptive method. The results showed that trigona honey had significantly different pH, water content, and reduced sugar content. The pH of trigona honey obtained from 6 farms in East Lombok Regency is in the general pH range of trigona honey, which ranges from 2.99 to 3.33. Meanwhile, the water content of trigona honey obtained from 6 farms in East Lombok Regency was very high, namely 27.98 - 41.01% (bb) with very low reducing sugar levels, which only ranged from 25.22 - 30.92%. However, the antimicrobial activity of trigona honey from 6 breeders in East Lombok showed the inhibition activity to Pseudomonas aeruginosa is relatively high that indicated by the formation of an inhibition zone due to the existence of flavonoid content in trigona honey as an antimicrobial agent.

References

Achyani dan Wicandra, D., 2019. Kiat Praktis Budidaya Lebah Trigona (Heterotrigona itama). CV. Laduny Alifatama. Lampung.

Adityarini, D., S.A.W. Suedy, dan S. Darmayanti, 2020. Kualitas Madu Lokal Berdasarkan Kadar Air, Gula Total dan Keasaman dari Kabupaten Magelang. Jurnal Anatomoni dan Fisiologi. 5(1): 18-24.

Badan Pusat Statistik (BPS), 2020. Produksi Madu Nasional.https://databoks.katadata.co.id.produksimadunasional. Diakses pada tanggal 16 September 2021.

Badan Pusat Statistik (BPS), 2021. Kabupaten Lombok Timur Dalam Angka 2021. BPS Kabupaten Lombok Timur. Selong.

Badan Standar Nasional (BSN), 2018. SNI 01-8664- 2018, Madu. Jakarta.

Boorn, K. L., Y. Y. Khor, E. Sweetman, F. Tan, T. A. Heard dan K. A. Hammer, 2009.

Antimicrobial Activity of Honey from The Stingless Bee Trigona carbonaria Determined by Agar Diffusion, Agar Dilution, Broth Microdilution and Time-kill Methodology. Journal of Applied Microbiology. 108(2010) : 1534-1543.

Cahyadi, M. A., Sidharta, B. B. R., & To, N. 2019. Karakteristik dan EfektivitasSalepMaduKlancengdariLebahTrigona sp. sebagaiAntibakteri dan Penyembuh Luka Sayat. Jurnal Biota. 4(3):104–109.

Chayati, I., 2008. Sifat Fisikokimia Madu Monoflora dari Daerah Istimewa Yogyakarta dan Jawa Tengah. Jurnal Agritech. 28(1): 9-14.

Chuttong, B., Y. Chanbang, K. Sringarm dan M. D. Burgett, 2016. Physicochemical Profiles of Stingless Bee (Apidae: Meliponini) Honey from South East Asia (Thailand). Journal of Food Chemistry. 192: 149-155.

Crane, E. 1979. Honey A Comprehensive Survey. The International Bee Research.

Darmawijaya, I. P., dan N. L. G. A. N. Yudha, 2013. Skrining Fitokimia Ekstrak Etanol Daun Pancasona (Tinosporacoriaceae Beumee.). 381–385.

Devianti, R. P., E. S. Soetarto, dan K. A. Hendarto, 2015. Komposisi dan Kandungan Bakteri Pada Madu Trigona sp. dan Aktivitas Antimikrobia Terhadap Mikrobia Patogen. Thesis S2 Biology. Universitas Gajah Mada. Yogyakarta.

Emmasitah, Raya, I., Usman, A.N., Mauludiyah, I., dan Prihartini, A.R., 2020. Uji FTIR Dan Uji Fitokimia Dari Madu Trigona Spp. Untuk Persiapan Suplemen Wanita Prakonsepsi. Jurnal Keperawatan Muhammadiyah. Edisi khusus. 1-6

Evahelda, E., Filli, P., Malahayati, N., & Santoso, B. 2017. Sifat fisik dan kimia madu dari nektar pohon karet di Kabupaten Bangka Tengah, Indonesia. Jurnal Agritech. 37(4): 363–368.

Fatma, I. I., S. Haryanti, dan S. W. A. Suedy, 2017. Uji kualitas madu pada beberapa wilayah budidaya lebah madu di Kabupaten Pati. Jurnal Biologi. 6(2): 58-65

Fauzy, A., 2019. Metode Sampling. Universitas Terbuka. Banten.

Gariola, A., P. Tiwari dan J.K. Tiwari, 2013. Physico-chemical Properties Of Apis Cerana Indica Honey From Uttarkashi District Of Uttarakhand. Jurnal of Global Biosciences. 2(1): 20-25.

Hadiwiyoto, S., 1993. Teknologi Pengolahan Hasil Perikanan. Penerbit Liberty. Yogyakarta.

Hakim, S. S., Siswandi, Wahyuningtyas, R.S., Rahmanto, B., Halwany W., dan Lestari, F., 2018. Sifat Fisikokimia dan Kandungan Mikronutrien pada Madu Kelulut (Heterotrigona itama) Dengan Warna Berbeda. Jurnal Penelitian Hasil Hutan. 39 (1): 1 – 12.

Hariyati, L. F., Andriani, M., dan Utami, R., 2010. Aktivitas Antibakteri Berbagai Jenis Madu Terhadap Bakteri Pembusuk (Pseodomonas fluorescens FNCC0071 dan Pseudomonas putida FNNCC0070). Jurnal Jurusan Teknologi Hasil Pertanian UNS: 1 – 9.

Harjanto, S., Mujianto, M., Arbainsyah dan Ramlan, A., 2020. Budidaya Lebah Madu Kelulut Sebagai Alternatif Mata Pencaharian Masyarakat. Yayasan Swaraowa. DI Yogyakarta.

Hassanain, A.T., Alyaa, A. K., dan Karim, A. J., 2010. Antimicrobial Effect of Malaysian Honey on Some Human Pathogens: An In Vitro Study. The International Medical Journal Malaysia. 9(2) : 15 – 18.

Hendra, R, S. Ahmad, A. Sukari, M.Y. Shukor, E. Oskoueian, 2011. Flavanoid analyses and Antimocrobial Activity of Various Parts of Phaleria Macrocarpa (Scheff.) Boerl Fruit. Int. J Mol Sci. 12: 3422-3431.

ICMSF (International Comission on Microbiological Specifications for Foods). 2005. Sugar, Syrups, and Honey. P. 522-543. In: Microorganisms in Foods 6: Microbial Ecology of Food Commodities, 2nd Edition. Kluwer Academic. New York.

Ikalinus, R., S. Widyastuti, dan S. N. Eka, 2015. Skrining Fitokimia Ekstrak Etanol Kulit Batang Kelor (Moringa Oleifera). Jurnal Indonesia Medicus Veterinus. 4(1): 71–79.

Imam, P.N., 2017. Pengaruh Suhu Fermentasi Pada Beberapa Varietas Bawang Putih Terhadap Mutu Black Garlic. Skripsi. Universitas Mataram. Mataram.

International Honey Commission, 2009. Harmonised methods of the International Honey Commission. 1-63. Swiss Bee Research Centre. Bern.

Korošec, M., R. Vidrih, J. Bertoncelj. 2017. Slovenian Honey and Honey Based Products. Ch. 7. In: R. M. S. Cruz and M. C. Vieira (Eds.). Mediterranean Foods: Composition and Processing. CRC Press, Boca Raton, FL.

Kumar, T., D.K. Tripathi, K. Nagori, S. Pure, dan S. Agrawal, 2011. Isolasion and antibacterial susceptibility testing of multi drug resistant Pseudomonas aeroginosa causing urinary tract infections. J Chem Pharm Res.

Kwapong, P. K., Aidoo, K., Combey, R., dan Karokari, A., 2010. Stingless Bees: Importance, Management and Utilisation: A Training Manualfor Stingless Bee Keeping.Ebook.https://www.researchgate.net/publication.

Ma’ruf, M., Mawaddah, G. A., Eriana, N. N. A., Swari, F. I., Aslamiah, S., dan Lutpiana, L., 2018. Madu Lebah Kelulut (Trigona Spp.) Dalam Aktivitas Terhadap Bakteri Staphylococcus aureus Resisten. Jurnal Skala Kesehatan. Politeknik Kesehatan Banjarmasin. 9(1).

Mahmood, A.L., M. N. Lani, Z. Hassan, S.B.A. Razak, dan F.T. Ahmad, 2021. Antioxidant and Antimicrobial Properties of Indo- Malayan Stingless Bee (Heterotrigona itama) Honey From Different Seasons and Distribution of Flowers. Journal Food Research. 5(2): 498-507.

Malaysian Standards (MS), 2017. Kelulut (stingless bee) honey–specification MS 2683. Department of Standards Malaysia.

Mizumoto, K., Sasaki, H., Kume, H. & Yamaguchi, M. Nutritional compositions for controlling blood glucose levels. European Patent Number EP142407A1 (2004).

Mundo, M.A., Olga I. Padilla-Zakour, and R.W. Worobo, 2004. Growth Inhibition of Food Pathogens and Food Spoilage Organisms by Selected Raw Honeys. International Journal of Microbiology. 97 : 1-8.

Munte, L., Runtuwene, M. R., & Citraningtyas, G. (2015). Aktivitas antioksidan dari ekstrak daun prasman (Eupatorium triplinerve Vahl.). Pharmacon, 4(3), 41–50.

Nadhilla, N. F., 2014. The Activity of Antibacterial Agent of Honey Against Staphylococcus aureus. Jurnal Majority. 3(7): 94-101.

Nur, A., dan S. Sirajuddin, 2019. Aktivitas Antibakteri Madu Trigona Terhadap Bakteri Gram Positif (Staphylococcus Aureus) dan Bakteri Gram Negatif (Escherichia Coli). Jurnal Kesehatan. 12(1): 134-140.

Priawandiputra, W., M. G. Azizi, Rismayanti, dan K. M. Djakaria, A. Wicaksono, R. Raffiudin, T. Atmowidi dan D. Buchori, 2020. Panduan Budidaya Lebah Tanpa Sengat (Stingless Bees) di Desa Perbatasan Hutan Studi di Lubuk Bintialo dan Pangkalan Bulian, Sumatera Selatan. Ebook ZSL Let’s Work For Wildlife.

Riendriasari, S. D., dan Krisnawati, 2017. Produksi Propolis Mentah Lebah Madu Trigona spp di Pulau Lombok. Jurnal HutanTropik. 1(1): 71-75.

Samadi, B., 2004. Budidaya Lebah Madu. Aneka Ilmu. Semarang.

Shahjahan, M., Halim, and N.A.B., Inzani, N.Z.N., 2007. Antimicrobial Properties of 'Kelulut' (Trigona Spp) Honey. Malaysian Journal of Medical Sciences . 14 (1): 107-108.

Simaremare, E.S., 2014. Skrining Fitokimia Ekstrak Etanol Daun Gatal (Laportea decumana (ROXB) WEDD). Jurnal Pharmacy. 11(1).

Sousa, J. M., Marques, G., Benassi, M. T., Gullόn, B., Pintado, M. M., Magnani, M. 2016. Sugar profile, physicochemical and sensory aspects of monofloral honeys produced by different stingless bee species in Brazilian semiarid region. Journal LWT-Food Science and Technology. 65: 645-651.

Souza, B. A., Roubik, D., Barth, O. M., Heard, T., 2006. Composition of Stingless Bee Honey: Setting Quality Standards. Interciencia. 31(12): 867-875.

Sudarmadji, S., Haryono, B., dan Suhardi, 1989. Analisa Bahan Makanan dan Pertanian. Penerbit Liberty. Yogyakarta.

Sudarmadji, S. Haryono, B., dan Suhardi, 2007. Analisis Bahan Makanan dan Pertanian. Liberty. Yogyakarta.

Sudarmadji, S. Haryono, B., dan Suhardi, 2010. Analisis Bahan Makanan dan Pertanian. Liberty. Yogyakarta.

Syafrizal, R. R., I. W. Kusuma, S. Egra, K. Shimizu,

M. Kanzaki dan E. T. Arung, 2020. Diversity and honey properties of stingless bees from meliponiculture in east and north Kalimantan, Indonesia. Biodiversitas. 21(10): 4623-4630.

Syaifudin, S. M., dan S. Normagiat, 2020. Budidaya Pakan Lebah Trigona sp. dengan Agroforestry System di Kelurahan Mempawah. Jurnal Ilmiah Pangabdhi. 6(1): 17-24.

Tanjung, R.A., R. Moulana dan S. Rasnovi, 2022. Pengaruh Keragaman Sumber Pakan Terhadap Kualitas Madu Lebah (Apis cerana Fabr, 1798) di Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan (BP2LHK) Aek Nauli Sumatera Utara. Jurnal Ilmiah Mahasiswa Pertanian. 7(1): 819-832.

Thohari, I., Mustakim, M. C. Padaga, P. P. Rahayu, 2017. Teknologi Hasil Ternak. UB Press. Malang.

Triwanto, J., K. Herlinda, dan T. Muttaqin, 2021. Kualitas Fisikokimia pada Madu dari Nektar Bunga Randu (Ceiba pentandra) dan Kaliandra (Calliandra callothyrsus). Jurnal of Forest Science Avicennia. 4(2) : 102- 113.

Tunny, R., Mahulauw, M. A. H., &Darmanta, K. 2020. Identifikasi Kandungan Senyawa Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Metanol Daun Asam Jawa (Tamarindus Indica L.) Kecamatan Kairatu Kabupaten Seram Bagian Barat. Jurnal2- TRIK: Tunas-Tunas Riset Kesehatan. 10(1): 1–5.

Ulfa, M., 2020. Perbedaan Kualitas Madu LebahTrigona sp. dari Beberapa Wilayah di Pulau Lombok. Thesis S1. Universitas Mataram. Mataram.

Wahyuni, N., S. D. Riendriasari, R. A. Hasan, M. Hidayatullah, C. Handoko, L. Anggadhania dan Yamantoko, 2020. Produk Perlebahan NTB, Fakta dan Strategi Pengelolaan. CV. Nas Media Pustaka. Makasar.

Yuliana, R., Sutariningsih, E., Santoso, H. B., Hendarto K. A., dan Riendrasari,S. D., 2015. Daya Antimikrobia Sarang Lebah MaduTrigona spp. Terhadap Mikrobia Patogen. Jurnal Bioedukasi. 8(1): 67 – 72.

Zahra, N. N., H. Muliasari, Y. Andayani, dan I. M. Sudarma, 2021. Karakteristik Fisikokimia Ekstrak Madu dan Propolis Trigona sp. Asal Lombok Utara. Jurnal Agrotek Ummat. 8(1).

Zulhawa, D. J., 2010. Daya Hambat Madu Sumbawa Terhadap Pertumbuhan Kuman Staphylococcus aureus Isolat Infeksi Luka Operasi RS Islam Amal Sehat Sragen. Skripsi. Fakultas Kedokteran, Universitas Sebelas Maret. Surakarta.

Published

2023-05-30

How to Cite

Hifzi Ulan Nasri, S. ., Widyastuti, S. ., & Ariyana, M. D. (2023). KAJIAN MUTU KIMIA DAN DAYA HAMBAT MADU LEBAH TRIGONA (Tetragonula clypearis) DARI PETERNAKAN DI KABUPATEN LOMBOK TIMUR TERHADAP Pseudomonas aeruginosa SECARA IN VITRO: Study On Chemical Quality And Inhibitory Efficacy Of Trigona Bee (Tetragonula Clypearis) Honey From Breeders In East Lombok District Against Pseudomonas Aeruginosa Using In Vitro Analysis. Pro Food, 9(1), 12–23. https://doi.org/10.29303/profood.v9i1.289

Most read articles by the same author(s)

<< < 1 2