Exploration of Starch from Cassava Peel Waste as an Alternative Carbon Source for the Growth Medium of Rhizopus oligosporus Saito
DOI:
https://doi.org/10.29303/profood.v11i1.513Keywords:
Kulit singkong, media pertumbuhan, Rhizopus oligosporus SaitoAbstract
Cassava (Manihot esculenta Crantz) is an important staple food for around 800 million people, mainly in tropical regions, with global production increasing by around 100 million tons since 2000. In Indonesia, increased cassava consumption also generates cassava peel waste, which is generally discarded or used as low economic value animal feed. Poorly managed disposal can lead to environmental pollution and methane gas emissions. Cassava peel, with a starch content of up to 36%, has the potential as an alternative medium for microorganism growth that is cheaper than commercial media such as Potato Dextrose Agar (PDA). This study aims to utilize cassava peel waste as a growth medium for Rhizopus oligosporus Saito, a fungus used in food fermentation and capable of producing high-value secondary metabolites. So it is necessary to develop alternative media that are efficient and environmentally friendly for microbial growth. The research methods included characterization of R. oligosprus Saito, inoculum preparation, cassava peel starch powder preparation, and optimization of cassava peel starch concentration, glucose, and media pH using Submerged Fermentation (SmF). The results showed that R. oligosprus Saito can grow on cassava peel-based alternative media with optimum conditions at 40 g/L cassava peel starch, 10 g/L glucose, and pH 6 – 6.5. This research shows the potential of cassava peel as an economical and environmentally friendly carbon source, while supporting the development of biotechnology products based on organic waste.
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