Identifikasi Kandungan Daging Sapi Menggunakan PCR Pada Pedagang Bakso Tusuk Keliling Di Lingkungan Kota Mataram

Identification Of Beef Content Using PCR In Bakso Tusuk Roves Traders In The Mataram City Environment

Authors

  • Lalu Unsunnidhal Fakultas Teknologi Pangan dan Agroindustri, Universitas Mataram
  • Firman Fajar Perdhana Universitas Mataram
  • Qabul Dinanta Utama Universitas Mataram
  • Raudatul Jannah STIKES Yarsi Mataram

DOI:

https://doi.org/10.29303/profood.v9i1.312

Keywords:

Bakso tusuk, Kota Mataram, PCR, prime

Abstract

Daging merupakan sumber protein penting dalam kehidupan sehari-hari manusia. Selain itu, dengan gaya hidup perkotaan yang sibuk dan padat, makanan olahan kini menjadi salah satu sumber protein utama dalam menu makanan seseorang. Konsumen bergantung pada pelabelan makanan untuk memutuskan apakah produk daging yang mereka beli aman dan terpercaya. Oleh karena itu, penting untuk memastikan pelabelan makanan dilakukan dengan cara yang benar untuk menghindari penipuan konsumen. Lebih banyak konsumen saat ini yang peduli dengan kualitas dan keamanan makanan daripada sebelumnya. Penelitian ini memaparkan metode untuk mengidentifikasi dan mendeteksi kandungan daging sapi menggunakan Polymerase Chain Reaction (PCR) pada 8 produk bakso tusuk dari pedagang keliling di sekitar Kota Mataram, Provinsi Nusa Tenggara Barat. Tujuan dari penelitian ini adalah untuk mengetahui kandungan daging sapi yang digunakan pada produk yang diuji. Temuan penting utama dalam karya ini adalah spesifisitas primer oligonukleotida untuk daging sapi olahan dan penentuan kandungan daging sapi pada bakso tusuk di sekitar Kota Mataram, Provinsi Nusa Tenggara Barat.

References

Aida, A. et al. (2005). Analysis Of Raw Meats And Fats Of Pigs Using Polymerase Chain Reaction For Halal Authentication. Meat Science, 69, pp. 47–52.

Ding, H. and Xu, R. (1999). Differentiation of Beef and Kangaroo Meat by Visible/Near-Infrared Reflectance Spectroscopy. Journal of Food Science, 64, pp. 814–817.

Fathurrahman, I. et al. (2022). Molecular Sexing In Bos Taurus Using Quantitative Polymerase Chain Reaction (Qpcr) Method. IOP Conference Series: Earth and Environmental Science, 976(1). doi: 10.1088/1755-1315/976/1/012002.

Fihiruddin, F. et al. (2022). Expression And Epitope Prediction Of MPT64 Recombinant Proteins From Clinical Isolates Of Mycobacterium Tuberculosis As Immunoserodiagnostic Candidates. Veterinary World, 15(2), pp. 2376–2383. doi: ww.doi.org/10.14202/vetworld.2022.2376-2383.

Girish, P. et al. (2004). Sequence Analysis Of Mitochondrial 12S Rrna Gene Can Identify Meat Species. Meat Scien, 66, pp. 551–556.

Hird, H., Goodier, R. and Hill, M. (2003). Rapid Detection Of Chicken And Turkey In Heated Meat Products Using The Polymerase Chain Reaction Followed By Amplicon Visualisation With Vistra Green. Meat Science, 65, pp. 1117–1123.

Ishak, J. et al. (2019). In vitro evaluation of chitosan-DNA plasmid complex encoding Jembrana disease virus Env-TM protein as a vaccine candidate. Journal of Veterinary Research, 63(1), pp. 7–16. doi: https://doi.org/10.2478/jvetres-2019-0018.

Jannah, R. and Unsunnidhal, L. (2019). Konstruksi Dan Kloning Plasmid Pcdna3 . 1 ( + ) Dengan Subgenotip B3 Hepatitis B Core Antigen ( Hbcag ) Sebagai Kandidat Vaksin Dna Hepatitis B. Jurnal Penelitian dan Kajian Ilmiah Kesehatan, 5(2), pp. 125–131.

Kocher, T. et al. (1989). Dynamics Of Mitochondrial DNA Evolution In Animals: Amplification And Sequencing With Conserved Primers. Proceedings of the National Academy of Sciences, 86, pp. 6196–6200.

Mane, B., Mendiratta, S. and Tiwari, A. (2009). Polymerase Chain Reaction Assay For Identification Of Chicken In Meat And Meat Products. Food Chemistry, 116, pp. 806–810.

Martin, I. et al. (2007). Mitochondrial Markers For The Detection Of Four Duck Species And The Specific Identification Of Muscovy Duck In Meat Mixtures Using The Polymerase Chain Reaction. Meat Science, 76, pp. 721–729.

Matsunaga, T. et al. (1999). A Quick And Simple Method For The Identification Of Meat Species And Meat Products By PCR Assay. Meat Science, 51, pp. 143–148.

Nakyinsige, K., Man, Y. and Sazili, A. (2012). Halal Authenticity Issues In Meat And Meat Products. Meat science, 91(3), pp. 207–214.

Ong, S. et al. (2007). Meat Molecular Detection: Sensitivity Of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism In Species Differentiation Of Meat From Animal Origin. ASEAN Food Journal, 14, pp. 51–59.

Pascoal, A. et al. (2004). Survey Of Authenticity Of Meat Species In Food Products Subjected To Different Technological Processes, By Means Of PCR-RFLP Analysis. European Food Research and Technology, 218, pp. 306–312.

Peter, C. et al. (2004). Ifferentiation Of Animal Species In Food By Oligonucleotide Microarray Hybridization. European Food Research and Technology, 219, pp. 286–293.

Rahman, A. et al. (2022). Molecular Verification of Sex-separated Straw of Simmental Cattle ( Bos taurus ) by Polymerase Chain Reaction (PCR). Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021), 18(Istap 2021), pp. 223–226. doi: 10.2991/absr.k.220207.046.

Rao, Q. and Hsieh, P. (2007). Evaluationofa Commercial Lateral flowfeedtest Forrapid Detection Of Beef And Sheep Content In Raw And Cooked Meats. Meat Science, 76, pp. 489–494.

Sahilah, A. et al. (2012). Comparison Between Pork And Wild Boar Meat (Sus Scrofa) By Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Sains Malaysiana, 41, pp. 199–204.

Unsunnidhal, L., Jannah, R., et al. (2021). Potential of Nanoparticles Chitosan for Delivery pcDNA3.1-SB3- HBcAg. BIO Web of Conferences, 41(07003), pp. 1–6.

Unsunnidhal, L., Wasito, R., Setyawan, E. M. N., et al. (2021). Potential of Nanoparticles Chitosan for Delivery pcDNA3.1-tat. BIO Web of Conferences, 41(07004), pp. 1–6.

Unsunnidhal, L., Wasito, R., Nugraha Setyawan, E. M., et al. (2021). Potential of polylactic-co-glycolic acid (PLGA) for delivery Jembrana disease DNA vaccine Model (pEGFP-C1-tat). Journal of Veterinary Science, 22(6), pp. 1–15. doi: 10.4142/jvs.2021.22.e76.

Unsunnidhal, L., Ishak, J. and Kusumawati, A. (2019). Expression of gag-CA Gene of Jembrana Disease Virus with Cationic Liposomes and Chitosan Nanoparticle Delivery Systems as DNA Vaccine Candidates. Tropical Life Sciences Research, 30(3), pp. 15–36. doi: https://doi.org/10.21315/tlsr2019.30.3.2.

Unsunnidhal, L. and Jannah, R. (2019). Potential Of Cationic Liposomes And Chitosan Nanoparticles For Delivery Dna Vaccine Model Ntc8685-Egfp. Jurnal Penelitian dan Kajian Ilmiah Kesehatan, 5(2), pp. 120–124.

Wissiack, R. et al. (2003). Screening Test To Detect Meat Adulteration Through The Determination Of Hemoglobin By Cation Exchange Chromatography With Diode Array Detection. Meat Science, 64, pp. 427–432.

Published

2023-05-30

How to Cite

Unsunnidhal, L., Perdhana, F. F. ., Utama, Q. D. ., & Jannah, R. . (2023). Identifikasi Kandungan Daging Sapi Menggunakan PCR Pada Pedagang Bakso Tusuk Keliling Di Lingkungan Kota Mataram: Identification Of Beef Content Using PCR In Bakso Tusuk Roves Traders In The Mataram City Environment. Pro Food, 9(1), 76–81. https://doi.org/10.29303/profood.v9i1.312