Construction and Cloning of the CelE Gene Encoding the Cellulase Enzyme as A Candidate Enzyme For the Food and Agroindustry


  • Sukarne Universitas Mataram
  • Hasma Universitas Mataram
  • Raudatul Jannah STIKES Yarsi Mataram
  • Muhammad Aidil Fitriyan Fadjar Suryadi Universitas Mataram
  • Lalu Unsunnidhal Universitas Mataram



CelE, Cellulase Enzyme, Recombinant DNA Cloning, Recombinant DNA Construction


Research on the construction and cloning of the CelE gene, which encodes the cellulase enzyme from Ruminococcus plavefaciens, emerged as a response to the urgent need for efficient enzyme resources in the food industry and agro-industry. Cellulase enzymes, with a focus on CelE, have a vital role in the degradation process of cellulose, a main component in plant cell walls. The existence of Ruminococcus plavefaciens as a source of the CelE gene is of interest because this microorganism is found in the digestive system of ruminant animals and has great potential to produce efficient cellulase enzymes. This research aimed to create and clone the pET15b plasmid with the CelE gene. Confirmation of the CelE gene in recombinant DNA was carried out by identifying host bacterial resistance in media with antibiotics and colony PCR. This research was carried out by applying the following methods: Implementation of Codon Optimization and Recombinant Plasmid Construction (CelE), Preparation of Competent Cells, Cell Transformation, and Transformant Colony PCR Test. The results of the research show that this research has succeeded in obtaining the expected transformant bacteria with the results of the Transformant Colony PCR Test. obtained the appropriate product size, namely 205 bp, so it can be concluded that the pET15b plasmid with the optimized CelE gene was successfully constructed and cloned.


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How to Cite

Sukarne, Hasma, Jannah, R., Suryadi, M. A. F. F., & Unsunnidhal, L. (2024). Construction and Cloning of the CelE Gene Encoding the Cellulase Enzyme as A Candidate Enzyme For the Food and Agroindustry. Pro Food, 10(1), 30–39.