Microbiological Quality and Sensory Characteristics of Sambal Petis from Fried Snack Vendors in Tambaksari District, Surabaya, Indonesia

Authors

  • Aulia Islamiati Yusuf Pangan Universitas Pembangunan Nasional Veteran Jawa Timur
  • Sri Winarti Universitas Pembangunan Nasional Veteran Jawa Timur
  • Destiana Adinda Putri Universitas Pembangunan Nasional Veteran Jawa Timur
  • Anna Abdilla Universitas Pembangunan Nasional Veteran Jawa Timur
  • Rosyid Wardianto Universitas Pembangunan Nasional Veteran Jawa Timur
  • Maulia Widi Universitas Pembangunan Nasional Veteran Jawa Timur

DOI:

https://doi.org/10.29303/profood.v12i1.639

Keywords:

Hygiene sanitation, microbial contamination, sambal petis, sensory evaluation, street vendors

Abstract

Sambal petis is a traditional Indonesian condiment that is frequently consumed with fried snacks. Nevertheless, the risk of microbial contamination may be elevated by an insufficient level of sanitation during the preparation and handling processes. This study aimed to assess sanitation practices, microbiological quality, and sensory characteristics of sambal petis from 26 fried-snack vendors in Tambaksari, Surabaya, Indonesia. Microbiological quality was assessed based on compliance with applicable microbiological standards, while sensory attributes were analyzed using one-way ANOVA followed by Tukey’s HSD test (p < 0.05). In general, sanitation during processing and equipment was moderate (mean score = 2), while food presentation and vending facilities sanitation were poor (mean score = 3). The total microbial count ranged from 5.17 to 6.25 log CFU/g, exceeding the maximum limit specified in SNI 2718:2013 of 3.69 log cfu/g. Furthermore, E. coli was identified in 50% of the samples, while Salmonella sp. Was identified in 46% of the samples. These results correspond to the observed hygiene behaviors, indicating that microbial contamination may be an implication of inadequate sanitation. Sensory evaluation indicated no significant differences for color, whereas texture and aroma varied significantly among samples. These findings emphasize the importance of effective hygiene, sanitation, and microbiological control measures throughout the food handling chain.

References

Adefrash, K., Sharew, B., Amare, W., & Shibabaw, A. (2024). Bacterial foodborne illness and Escherichia coli O157: H7 strain infection among asymptomatic food handlers in Northeast Ethiopia: Implication for hygienic practices and mass‐screening. Health Science Reports, 7(6), e2199.

Amaami, A. J., Dominic, D., & Collins, D. (2017). Factors associated with poor food safety compliance among street food vendors in the Techiman Municipality of Ghana. African Journal of Food Science, 11(3), 50–57.

Andrade, A. A., Paiva, A. D., & Machado, A. B. F. (2023). Microbiology of street food: understanding risks to improve safety. Journal of Applied Microbiology, 134(8), lxad167.

Badan Pengawas Obat dan Makanan. (2024). Kilas Balik 2024: BBPOM Surabaya Perkuat Sistem Kewaspadaan Kasus Keracunan Obat dan Makanan.

Badan Standarisasi Nasional. (2013). Syarat Mutu dan Keamanan Pangan Petis Udang (SNI 2718.1:2013).

Bekhit, A. E.-D. A., Holman, B. W. B., Giteru, S. G., & Hopkins, D. L. (2021). Total volatile basic nitrogen (TVB-N) and its role in meat spoilage: A review. Trends in Food Science & Technology, 109, 280–302.

Belina, D., Hailu, Y., Gobena, T., Hald, T., & Njage, P. M. K. (2021). Prevalence and epidemiological distribution of selected foodborne pathogens in human and different environmental samples in Ethiopia: a systematic review and meta-analysis. One Health Outlook, 3(1). https://doi.org/10.1186/s42522-021-00048-5

Billah, M. M., & Rahman, M. S. (2024). Salmonella in the environment: A review on ecology, antimicrobial resistance, seafood contaminations, and human health implications. Journal of Hazardous Materials Advances, 13, 100407.

Deblais, L., Ahmedo, B. U., Ojeda, A., Mummed, B., Wang, Y., Mekonnen, Y. T., Demisie Weldesenbet, Y., Hassen, K. A., Brhane, M., McKune, S., Havelaar, A. H., Liang, S., & Rajashekara, G. (2024). Assessing fecal contamination from human and environmental sources using Escherichia coli as an indicator. Frontiers in Public Health, 12. https://doi.org/10.3389/fpubh.2024.1484808

Ebrahim, H., & Wondimagegn, M. K. (2025). Assessment of microbiological quality of some selected street vended foods, vendor’s safety practices, knowledge, and attitudes in Dessie Town, Ethiopia. Scientific Reports, 15(1). https://doi.org/10.1038/s41598-025-20971-1

Ehuwa, O., Jaiswal, A. K., & Jaiswal, S. (2021). Salmonella, food safety and food handling practices. Foods, 10(5). https://doi.org/10.3390/foods10050907

Fatiqin, A., Novita, R., & Apriani, I. (2019). Pengujian Salmonella dengan menggunakan media SSA dan E. coli menggunakan media EMBA pada bahan pangan. Jurnal Indobiosains, 1(1).

Gambushe, S. M., Zishiri, O. T., & El Zowalaty, M. E. (2022). Review of Escherichia coli O157: H7 prevalence, pathogenicity, heavy metal and antimicrobial resistance, African perspective. Infection and Drug Resistance, 4645–4673.

He, Y., Wang, J., Zhang, R., Chen, L., Zhang, H., Qi, X., & Chen, J. (2023). Epidemiology of foodborne diseases caused by Salmonella in Zhejiang Province, China, between 2010 and 2021. Frontiers in Public Health, 11. https://doi.org/10.3389/fpubh.2023.1127925

Herawati, C., Endayani, H., Indragiri, S., Kristanti, I., Wahyuni, N. T., Dani, A. H., & Hikmawati, I. (2023). Sanitary Hygiene and Behavior of Food Handlers in the Presence of Escherichia coli Bacteria. Journal of Pure & Applied Microbiology, 17(4).

Imoisi, C., Ikpahwore, D. O., & Iyasele, J. U. (2024). Heating time effects on viscosity profiles. Singapore Journal of Scientific Research, 14(1), 13–23. https://doi.org/10.3923/sjsr.2024.13.23

Jongenburger, I., Reij, M. W., Boer, E. P. J., Gorris, L. G. M., & Zwietering, M. H. (2010). Factors influencing the accuracy of the plating method used to enumerate low numbers of viable micro-organisms in food. International Journal of Food Microbiology, 143(1–2). https://doi.org/10.1016/j.ijfoodmicro.2010.07.025

Jumiati, J., & Suprapti, Y. (2023). Performa Sensoris Petis Dari Kaldu Rajungan Dengan Penambahan Gula Aren Dan Kombinasi Tepung: Petis Sensory Performance From Crab Broth With Additions Palm Sugar And Flour Combination. Jurnal Miyang: Ronggolawe Fisheries and Marine Science Journal, 3(2), 53–57.

Kaddouri, M., El Alia, O. A., Zine‐eddine, Y., Alqahtani, A. S., Bouhrim, M., Galman, A., & Boutoial, K. (2025). Evaluation of food safety practices and microbiological quality of street foods in Marrakech, Morocco. Food Science & Nutrition, 13(6), e70322.

Kamboj, S., Gupta, N., Bandral, J. D., Gandotra, G., & Anjum, N. (2020). Food safety and hygiene: A review. International Journal of Chemical Studies, 8(2). https://doi.org/10.22271/chemi.2020.v8.i2f.8794

Kassaw, S., Damtew, M., & Geto, A. (2025). Assessment of knowledge, attitudes, and hygienic practices of meat handlers towards food safety working in Arbaminch town public food service establishments, Southern Ethiopia. Food and Humanity, 4, 100517.

Kementerian Kesehatan Republik Indonesia. (2011). Higiene Sanitasi Jasaboga (1096/MENKES/PER/VI/2011).

Kirchner, M. K. (2020). The Effect of Consumer Behaviors on Cross-Contamination While Preparing Meals in a Consumer Kitchen. North Carolina State University.

Li, Y., Yuan, L., Liu, H., Liu, H., Zhou, Y., Li, M., & Gao, R. (2023). Analysis of the changes of volatile flavor compounds in a traditional Chinese shrimp paste during fermentation based on electronic nose, SPME-GC-MS and HS-GC-IMS. Food Science and Human Wellness, 12(1), 173–182.

Mkangara, M. (2023). Prevention and control of human Salmonella enterica infections: An implication in food safety. International Journal of Food Science, 2023(1), 8899596.

Mora-Coto, D., Moreno-Vélez, P., Luna-Muñoz, J., Moreno-Campuzano, S., & Ontiveros-Torres, M. A. (2025). Intestinal and Extraintestinal Pathotypes of Escherichia coli Are Prevalent in Food Prepared and Marketed on the Streets from the Central Zone of Mexico and Exhibit a Differential Phenotype of Resistance Against Antibiotics. Antibiotics, 14(4), 406.

Nikiema, M. E. M., De La Gandara, M. P., Compaore, K. A. M., Ba, A. K., Soro, K. D., Nikiema, P. A., Barro, N., Sangare, L., & Weill, F. X. (2021). Contamination of street food with multidrugresistant Salmonella, in Ouagadougou, Burkina Faso. PLoS ONE, 16(6 June 2021). https://doi.org/10.1371/journal.pone.0253312

Nur, D. F. A., Yulistiani, R., Rosida, D. F., & Raharjo, D. (2022). Occurrences Salmonella sp. and Escherichia Coli in Bulk and Packaged Chicken Sausages in Surabaya, Indonesia. AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment), 6(2). https://doi.org/10.29165/ajarcde.v6i2.99

Nurwati, & Hasdar, M. (2021). Sifat organoleptik kue brownies dengan penambahan rumput laut (Eucheuma cottonii). Journal of Food Technology and Agroindustry, 3.

Pratadina, A., Darundiati, Y. H., & Dangiran, H. L. (2017). Hubungan Higiene Dan Sanitasi Dengan Kontaminasi Escherichia coli Pada Jajanan Pedagang Kaki Lima di Sekolah Dasar Kelurahan Pendrikan Lor, Semarang. Jurnal Kesehatan Masyarakat, 5(5), 502–513.

Putri, A. M., & Kurnia, P. (2018). Identifikasi keberadaan bakteri coliform dan total mikroba dalam es dung-dung. Media Gizi Indonesia, 13(1), 41. https://doi.org/10.20473/mgi.v13i1.41-48

Ricci, A., Martelli, F., Razzano, R., Cassi, D., Lazzi, C., Neviani, E., & Bernini, V. (2020). Service temperature preservation approach for food safety: microbiological evaluation of ready meals. Food Control, 115, 107297.

Risdayanti, R., Latif, U. T. A., & Wirawan, H. P. (2023). Deteksi keberadaan bakteri pengkontaminasi pangan Salmonella sp. pada telur. Filogeni: Jurnal Mahasiswa Biologi, 3 (3), 117–121.

Romadhon, T., Khoirina, A. D., Alvianto, D., Rahayu, L. F. R., Purba, N. S., & Muzdalifah, N. (2025). Karakteristik Petis berdasarkan Bahan Baku dan Penambahan Bahan Pangan yang Berbeda: Artikel review. Jurnal Ilmiah Pangan Halal, 7(2), 182–195.

Saifullah, Rahman, A., Roy, C. K., Akter, S., Chowdhury, S. R., & Dash, B. K. (2026). Assessment of Food Safety Knowledge, Practices, and Microbial Hazards in Street‐Vended Foods: A Case Study From Jashore Town, Bangladesh. Food Safety and Health, 4(1), 207–219.

Sarastani, D., Kusumanti, I., & Indriastuti, C. E. (2023). Uji penerimaan konsumen terhadap mutu organoleptik petis ikan Situbondo dengan metode uji kesukaan. Bioscientist: Jurnal Ilmiah Biologi, 11(1), 32–45.

Sari, M. J., Diachanty, S., Irawan, I., Pamungkas, B. F., & Zuraida, I. (2021). Karakteristik fisikokimia petis dari air rebusan ikan layang. Jurnal Pascapanen Dan Bioteknologi Kelautan Dan Perikanan, 16(2). https://doi.org/10.15578/jpbkp.v16i2.759

Sheehama, W. L. N., & Singh, T. (2025). Food Safety in informal markets: How knowledge and attitudes influence vendor practices in Namibia. International Journal of Environmental Research and Public Health, 22(4), 631.

Surya, R., & Tedjakusuma, F. (2022). Diversity of sambals, traditional Indonesian chili pastes. In Journal of Ethnic Foods (Vol. 9, Issue 1). https://doi.org/10.1186/s42779-022-00142-7

Triani, A. T. (2025). Identifikasi Senyawa Volatil Menggunakan Teknik Gc-Ms Dan Profiling Sensori Petis Kupang Di Desa Balongdowo Kecamatan Candi Kabupaten Sidoarjo. Universitas Pembangunan Nasional" Veteran" Jawa Timur.

Ulyarti, U., Surhaini, S., Nazarudin, N., Ali, L., & Astrini, A. (2019). Physical properties of chilli sauce as affected by water yam starch concentration. Indonesian Food Science and Technology Journal, 2(2), 54–56. https://doi.org/10.22437/ifstj.v2i2.9466

Valeria, L. G., Sebastián, A. G., Gabriela, G. P., Montserrat, H. I., & Angélica, G. O. (2025). Evaluation of street food consumption patterns, vendor practices, and Salmonella enterica contamination in street tacos. International Journal of Gastronomy and Food Science, 39. https://doi.org/10.1016/j.ijgfs.2025.101112

World Health Organization. (2024). Food Safety.

Wu, J., Gong, S., Guo, Z., & Bai, L. (2024). Street food vendors’ hygienic and handling practices in China: Checklist development and observational assessment. Food Control, 166, 110765.

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Published

2026-05-31

How to Cite

Pangan, A. I. Y., Winarti, S., Putri, D. A., Abdilla, A., Wardianto, R., & Widi, M. (2026). Microbiological Quality and Sensory Characteristics of Sambal Petis from Fried Snack Vendors in Tambaksari District, Surabaya, Indonesia. Pro Food, 12(1), 45–58. https://doi.org/10.29303/profood.v12i1.639